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Exam skills · Papers 1 and 2

Scientific investigations

Every Life Sciences paper has an investigation question. Learn the exact wording for aims, hypotheses, variables, controls, reliability and validity, and practise on a fully worked example.

The vocabulary markers look for

TermMeaningHow to write it
AimWhat the investigation sets out to find“To investigate the effect of [independent] on [dependent].”
Investigative questionThe aim as a question“What is the effect of … on …?”
HypothesisA testable prediction linking the two variables“An increase in X will increase / decrease / have no effect on Y.”
Independent variableWhat the investigator deliberately changesUsually the x-axis
Dependent variableWhat is measuredUsually the y-axis; include how it is measured
Controlled (fixed) variablesFactors kept the same in all groupsName specific ones: temperature, volume, species, age
Control (group)A set-up without the factor being tested, for comparison“To show that the results are due to X only.”

Reliability, validity and ethics

Common mark losses: a hypothesis written as a question; naming “the amount” instead of a specific controlled variable; saying “to make it fair” without explaining; reaching a conclusion that goes beyond the data.

Worked example: light intensity and photosynthesis

Learners placed pondweed at different distances (10, 20, 30, 40 cm) from a lamp and counted the oxygen bubbles released per minute. Each distance was tested three times.

Distance (cm)Trial 1Trial 2Trial 3Average
1042384040
2030293130
3018222020
401191010
✏️ Model answers
1Independent variable: light intensity (distance from the lamp). Dependent: rate of photosynthesis (number of bubbles per minute).
2Two controlled variables: temperature of the water; the same species and length of pondweed (also CO₂ concentration).
3Hypothesis: an increase in light intensity will increase the rate of photosynthesis.
4Why three trials? To improve reliability — an average reduces the effect of errors.
5Conclusion: as light intensity increases (distance decreases), the rate of photosynthesis increases. The hypothesis is accepted.
6Why place a beaker of water between the lamp and the plant? To absorb heat from the lamp, so that temperature stays constant.

Planning an investigation

A “plan an investigation” question (often 5–7 marks) is marked on a checklist. Include:

  1. The apparatus and the groups (including a control).
  2. What will be changed and how (the independent variable, with specific values).
  3. What will be kept constant (at least two variables).
  4. What will be measured, how, and for how long.
  5. Repeats and sample size, for reliability.
  6. Safety and ethics where relevant.
0/8
Review the explanations above.
Answer in your exercise book, then open each memo to mark your work.
Question 1 · (2 marks)
Write a hypothesis for an investigation into the effect of temperature on the activity of the enzyme amylase.
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An increase in temperature will increase ✓ the activity of amylase up to an optimum temperature / or any testable prediction that links both variables ✓.
Question 2 · (3 marks)
In a study, 200 learners were given a new energy drink and 200 were given water. Their reaction times were measured. (a) Identify the control group. (b) Give ONE way the investigators made the results reliable. (c) Name ONE ethical consideration.
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(a) The learners given water ✓
(b) A large sample (200 per group) ✓
(c) Informed consent / the right to withdraw / confidentiality / no harm ✓
Question 3 · (6 marks)
Plan an investigation to determine the effect of fertiliser concentration on the growth of bean seedlings.
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Several groups of seedlings, each given a different fertiliser concentration (e.g. 0, 5, 10, 15 g·dm⁻³) ✓; a group with no fertiliser as control ✓; keep constant: the same species/age of seedling, volume of water, light, temperature, soil type ✓ (any two); measure height (or mass) after a fixed period, e.g. 3 weeks ✓; use many seedlings per group / repeat ✓; calculate averages and compare ✓
Question 4 · (3 marks)
A learner concluded from one trial that “plants grow better in red light”. Give TWO reasons why this conclusion may not be valid and ONE improvement.
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Only one trial / small sample, so results may not be reliable ✓; other variables may not have been controlled ✓. Improvement: repeat with more plants and control variables such as temperature and light intensity ✓.